Intravenous glucose tolerance test in Baluchi ewe lambs receiving dietary supplemental selenium-methionine and chromium-methionine

Document Type : Original Article

Authors
Amir Mousaie 1
Reza Valizadeh 2
Abbas Ali Naserian 2
Mohammad Heidarpour 3
Hossein Kazemi Mehrjerdi 3
1 Department of Animal Science, Faculty of Agriculture, University of Jiroft, Jiroft, Iran
2 Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
3 Department of Clinical Science, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
10.22034/bsr.2025.559992.1001
Abstract
The impact of dietary supplementation of 1.5 mg of Selenium/kg of diet as Selenium-methionine (Se-Met) and 0.8 mg of Chromium/kg of diet as Chromium-methionine (Cr-Met) and their combination (Se-Cr-Met) on glucose and insulin responses during intravenous glucose tolerance test (IVGTT) was examined on 24 Baluchi ewe lambs (18-20 weeks of age) with 6 replicates per treatment. Blood samples were collected before (time 0) and 2, 10, 20, 30, 45, 60, 90, 120 and 180 min after IVGTT. The supplementation of Se- and/or Cr-Met resulted in a lower peak glucose concentration at 2 minutes and a diminished concentration at 180 minutes following the infusion (P<0.05). Additionally, lambs receiving Cr-Met and Se-Cr-Met exhibited lower serum glucose levels at 120 minutes in comparison to the control group (P≤ 0.05). Furthermore, animals consuming diets supplemented with Se-Met and/or Cr-Met showed a reduced area under the curve (AUC) for glucose and insulin at 180 minutes following the IVGTT (P<0.05). The quantity of circulating malondialdehyde, which serves as an indicator of oxidative stress, was reduced in lambs supplemented with Se-Met and/or Cr-Met (P<0.05). It can be concluded that the supplementation of organic Se-Met and/or Cr-Met enhanced glucose clearance and insulin sensitivity in growing Baluchi ewe lambs, with these effects possibly mediated by a decrease in systemic oxidative stress.
Keywords
Chromium
Ewe lamb
Glucose tolerance test
Insulin
Selenium
Subjects
Abuelo, A., Alves-Nores, V., Hernandez, J., Muino, R., Benedito, J.L., Castillo, C., 2016. Effect of parenteral antioxidant supplementation during the dry period on postpartum glucose tolerance in dairy cows. Journal of Veterinary Internal Medicine, 30: 892-898.
Baumgard, L.H., Hausman, G.J., Sanz Fernandez, MV., 2015. Insulin: pancreatic secretion and adipocyte regulation. Domestic Animal Endocrinology, 54: 76-84.
Chalmeh, A., Pourjafar, M., Badiei, K., Mirzaei, A., Jalali, M., Mazrouei Sebdani, M., 2021. Effects of dietary antioxidants on glucose and insulin responses to glucose tolerance test in transition dairy cows. Domestic Animal Endocrinology, 75: 106602. DOI: 10.1016/j.domaniend.2020.106602.
Clarke, L., Firth, K., Heasman, L., Juniper, D.T., Budge, H., Stephenson, T. Symonds, ME., 2000. Influence of relative size at birth on growth and glucose homeostasis in twin lambs during juvenile life. Reproduction, Fertility and Development, 12: 69-73.
Emami, A., Ganjkhanlou, M., Zali, A., 2014. Effects of Cr methionine on glucose metabolism, plasma metabolites, meat lipid peroxidation, and tissue chromium in Mahabadi goat kids. Biological Trace Element Research, 164: 50-57.
Ezaki, O., 1990. The insulin-like effects of selenate in rate adipocytes. Journal of Biological Chemistry, 265: 1124-1128.
Gatford, K.L., De Blasio, M.J., Thavaneswaran, P., Robinson, J.S., McMillen, IC., Owens, J.A., 2004. Postnatal ontogeny of glucose homeostasis and insulin action in sheep. American Journal of Physiology-Endocrinology and Metabolism, 286: 1050-1059.
Hayirli, A., Bremmer, D.R., Bertics, S.J., Socha, M.T., Grummer, R.R., 2001. Effect of chromium supplementation on production and metabolic parameters in periparturient dairy cows. Journal of Dairy Science, 84: 1218-1230.
Iranian Council of Animal Care., 1995. Guide to the care and use of experimental animals. vol 1. Isfahan University of Technology, Isfahan, Iran.
Kegley, E.B., Spears, J.W., Brown, T.T., 1997. Effect of shipping and chromium supplementation on performance, immunity response, and disease resistance of steers. Journal of Animal Science, 75: 1956-1964.
Kitchalong, L., Fernandez, J.M., Bunting, L.D., Southern, L.L., Bidner, T.D., 1995. Influence of chromium tripicolinate on glucose metabolism and nutrient partitioning in growing lambs. Journal of Animal Science, 73: 2694-2705.
McNeill, J.H., Delgatty, H.L.M., Battell, M.L., 1991. Insulin-like effects of sodium selenate in streptozotocin-induced diabetic rats. Diabetes, 40: 1675-1679.
Mousaie, A., Valizadeh, R., Naserian, A.A., Heidarpour, M., Kazemi Mehrjerdi, H., 2014. Impacts of feeding selenium-methionine and chromium-methionine on performance, serum components, antioxidant status and physiological responses to transportation stress of Baluchi ewe lambs. Biological Trace Element Research, 162: 113-123.
Mousaie, A., Valizadeh, R., Chamsaz, M., 2017. Selenium-methionine and chromium-methionine supplementation of sheep around parturition: impacts on dam and offspring performance. Archives of Animal Nutrition, 71: 134-149.
Mousaie, A., 2021. Dietary supra-nutritional supplementation of selenium-enriched yeast improves feed efficiency and blood antioxidant status of growing lambs reared under warm environmental conditions. Tropical Animal Health and Production, 53: 138.
Qiao, K., Jiang, R., Contreras, G.A., Xie, L., Pascottini, O.B., Opsomer, G., Dong, Q., 2024. The Complex interplay of insulin resistance and metabolic inflammation in transition dairy cows. Animals, 14: 832. DOI: 10.3390/ani14060832.
Rahman, M.S., Hossain, K.S., Das, S., Kundu, S., Adegoke, E.O., Rahman, M.A., Hannan, M.A., Uddin, M.J., Pang, M.G., 2021. Role of insulin in health and disease: An update. International Journal of Molecular Sciences, 22: 6403. DOI: 10.3390/ jms22126403.
Ravelo, A.D., Matamoros, C., Harvatine, K.J., Salfer, I.J., 2025. Daily rhythms of glucose, insulin, and nonesterified fatty acid responses to an intravenous glucose tolerance test in dairy cows. Journal of Dairy Science, 108: 5462-5474.
Vincent, J.B., 2007. The nutritional biochemistry of chromium (Ш). Elsevier, First edition. 293 pp.
Vonnahme, K.A., Luther, J.S., Reynolds, L.P., Hammer, C.J., Carlson, D.B., Redmer, D.A., Caton, J.S., 2010. Impacts of maternal selenium and nutritional level on growth, adiposity, and glucose tolerance in female offspring in sheep. Domestic Animal Endocrinology, 39: 240-248.
Biospeices Research
Volume 1, Issue 1
Winter 2026
Pages 1-9

  • Receive Date 21 November 2025
  • Revise Date 13 December 2025
  • Accept Date 22 December 2025
  • First Publish Date 22 December 2025
  • Publish Date 01 February 2026